Metabolite Profiling of Urban and Forest Tree Rhizosphere Soil using Analytical Instruments
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Abstract
Abstract
The rhizosphere is the area of the soil affected by plant roots and plays an important role in plant health, nutrient cycling, and soil structure. This study investigated differences in rhizosphere soil metabolites between trees in urban and forest environments using LC/MS-Q-TOF and GC/MS. Urban environments can cause soil pollution and ecosystem disturbance due to the accumulation of heavy metals and petrochemicals, while forest environments are rich in organic matter and fallen leaves, maintaining a nutrient-rich environment in which various microorganisms coexist. Rhizosphere soil samples were collected from Dankook University (city) and Taejo Mountain (forest) in Cheonan, South Korea, extracted using methanol and chloroform using the Soxhlet extraction method, and then analyzed by LC/MS-Q-TOF and GC/MS. N-Nitrosopyrrolidine, a possible carcinogen from automobile exhaust and industrial activities, has been found in the rhizosphere soil of urban trees. Forest rhizosphere samples showed high concentrations of momilactone A, which is important for plant defense mechanisms. 140 significant metabolites were identified through MPP analysis. Furthermore, GC/MS analysis confirmed that siloxanes and oleamides were predominantly detected in urban and forest samples, respectively. Overall, this study highlights the impact of environmental conditions on rhizosphere soil chemistry and microbial ecosystems and provides insights for urban greening and forest conservation strategies. Comprehensive profiling of rhizosphere metabolites will improve our understanding of plant-soil interactions and contribute to maintaining soil health and plant diversity in diverse ecosystems.
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Berendsen RL, Pieterse CM, Bakker PA. The rhizosphere microbiome and plant health. Trends Plant Sci. 2012 Aug;17(8):478-86. Available from: https://pubmed.ncbi.nlm.nih.gov/22564542/
Hartmann M, Six J. Soil structure and microbiome functions in agroecosystems. Nat Rev Earth Environ. 2023;4(1):4-18. Available from: https://www.nature.com/articles/s43017-022-00366-w
Henneron L, Kardol P, Wardle DA, Cros C, Fontaine S. Rhizosphere control of soil nitrogen cycling: a key component of plant economic strategies. New Phytol. 2020;228(4):1269-1282. Available from: https://pubmed.ncbi.nlm.nih.gov/32562506/
Rosier CL, Polson SW, D'Amico V 3rd, Kan J, Trammell TLE. Urbanization pressures alter tree rhizosphere microbiomes. Sci Rep. 2021;11(1):9447. Available from: https://pubmed.ncbi.nlm.nih.gov/33941814/
Riyam NK, Amal IH, Hosam MS. Heavy Metal’s Environmental Impact. In: Hosam MS, Amal IH, editors. Environmental Impact and Remediation of Heavy Metals. Rijeka: IntechOpen; 2022. Chapter 1. Available from: https://www.intechopen.com/chapters/81083
Li FJ, Yang HW, Ayyamperumal R, Liu Y. Pollution, sources, and human health risk assessment of heavy metals in urban areas around industrialization and urbanization-Northwest China. Chemosphere. 2022;308(Pt 2):136396. Available from: https://pubmed.ncbi.nlm.nih.gov/36113648/
Ruehr NK, Buchmann N. Soil respiration fluxes in a temperate mixed forest: seasonality and temperature sensitivities differ among microbial and root-rhizosphere respiration. Tree Physiol. 2010; 30(2):165-76. Available from: https://pubmed.ncbi.nlm.nih.gov/20008837/
Cortesi I, Ferretti LV, Morgia F. Soil and Water as Resources: How Landscape Architecture Reclaims Hydric Contaminated Soil for Public Uses in Urban Settlements. Sustainability. 2020;12(21):8840. Available from: https://doi.org/10.3390/su12218840
Tolkkinen MJ, Heino H, Ahonen SHK, Lehosmaa K, Mykrä H. Streams and riparian forests depend on each other: A review with a special focus on microbes. For Ecol Manage. 2020;462:117962. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0378112719323394
Balla A, Sillini A, Hafsa Cherif-Silini, Ali Chenari Bouket, Justyna Anna Nowakowska, et al. The Threat of Pests and Pathogens and the Potential for Biological Control in Forest Ecosystems. Int J Mol Sci. 2021 Nov;12(11):1579. Available from: https://www.mdpi.com/1999-4907/12/11/1579
Silva LCR, Lambers H. Soil-plant-atmosphere interactions: structure, function, and predictive scaling for climate change mitigation. Plant Soil. 2021 Jan;461(1):5-27. Available from: https://link.springer.com/article/10.1007/s11104-020-04427-1
Maria Pellegrino R, Ianni F, Blasi F, Angelini P, Emiliani C, Venanzoni R, et al. Lipidomic profiling of Pleurotus ostreatus by LC/MS Q-TOF analysis. Food Res Int. 2022 Jun;156:111335. Available from: https://pubmed.ncbi.nlm.nih.gov/35651085/
Allen DR, McWhinney BC. Quadrupole Time-of-Flight Mass Spectrometry: A Paradigm Shift in Toxicology Screening Applications. Clin Biochem Rev. 2019;40(3):135-146. Available from: https://pubmed.ncbi.nlm.nih.gov/31530964/
Serb AF, Georgescu M, Onulov R, Novaconi CR, Sisu E, Bolocan A, et al. Mass-Spectrometry-Based Research of Cosmetic Ingredients. Molecules. 2024 Mar 17;29(6):1336. Available from: https://pubmed.ncbi.nlm.nih.gov/38542972/
Jung J-M, Lee TW, Jung S, Tsang YF, Bhatnagar A, Lee SS, et al. Control of the fate of toxic pollutants from catalytic pyrolysis of polyurethane by oxidation using CO2. Chem Eng J. 2022 Mar;442:136358. Link: Available from: https://www.sciencedirect.com/science/article/abs/pii/S1385894722018538
Chen Q, Yang X, Hong P, Liu M, Li Z, Zhou C, et al. GC-MS, GC-IMS, and E-Nose Analysis of Volatile Aroma Compounds in Wet-Marinated Fermented Golden Pomfret Prepared Using Different Cooking Methods. Foods. 2024;13(3):390. Available from: https://pubmed.ncbi.nlm.nih.gov/38338525/
Kunene PN, Mahlambi PN. Optimization and application of ultrasonic extraction and Soxhlet extraction followed by solid phase extraction for the determination of triazine pesticides in soil and sediment. J Environ Chem Eng. 2020 Apr;8(2):103665. Available from: https://www.sciencedirect.com/science/article/abs/pii/S2213343720300130
Koilybayeva M, Shynykul Z, Ustenova G, Waleron K, Jońca J, Mustafina K, et al. Gas Chromatography-Mass Spectrometry Profiling of Volatile Metabolites Produced by Some Bacillus spp. and Evaluation of Their Antibacterial and Antibiotic Activities. Molecules. 2023;28(22):7556. Available from: https://pubmed.ncbi.nlm.nih.gov/38005278/
Schwaiger M, Schoeny H, El Abiead Y, Hermann G, Rampler E, Koellensperger G. Merging metabolomics and lipidomics into one analytical run. Analyst. 2019 Jan;144(1):220-229. Available from: https://pubs.rsc.org/en/content/articlelanding/2019/an/c8an01219a
Ulmer CZ, Jones CM, Yost RA, Garrett TJ, Bowden JA. Optimization of Folch, Bligh-Dyer, and Matyash sample-to-extraction solvent ratios for human plasma-based lipidomics studies. Anal Chim Acta. 2018;1037:351-357. Available from: https://pubmed.ncbi.nlm.nih.gov/30292311/
Ketkar MB, Schneider P, Preussmann R, Plass Ch, Mohr U. Carcinogenic effect of low doses of nitrosopyrrolidine administered in drinking water to Syrian golden hamsters. J Cancer Res Clin Oncol. 1982;104(1-2):75-9. Available from: https://pubmed.ncbi.nlm.nih.gov/7130251/
Preussmann R, Schmähl D, Eisenbrand G. Carcinogenicity of N-nitrosopyrrolidine: dose-response study in rats. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1977;90(2):161-6. Available from: https://pubmed.ncbi.nlm.nih.gov/145756/
Lijinsky W, Taylor HW. The effect of substituents on the carcinogenicity of n-nitrosopyrrolidine in Sprague-Dawley rats. Cancer Res. 1976;36(6):1988-90. Available from: https://pubmed.ncbi.nlm.nih.gov/178438/
Kato-Noguchi H. Defensive Molecules Momilactones A and B: Function, Biosynthesis, Induction and Occurrence. Toxins. 2023;15(4):241. Available from: https://www.mdpi.com/2072-6651/15/4/241
Badri DV, Vivanco JM. Regulation and function of root exudates. Plant Cell Environ. 2009;32(6):666-81. Available from: https://doi.org/10.1111/j.1365-3040.2008.01926.x
Hartmann A, Schmid M, van Tuinen D, Berg G. Plant-driven selection of microbes. Plant Soil. 2009;321(1):235-257. Available from: https://link.springer.com/article/10.1007/s11104-008-9814-y
Kim SC, Chapman KD, Blancaflor EB. Fatty acid amide lipid mediators in plants. Plant Sci. 2010;178(5):411-419. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0168945210000476
Li P, Wang S, Liu M, Dai X, Shi H, Zhou W, et al. Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against Ralstonia pseudosolanacearum. Plants (Basel). 2024;13(4):482. Available from: https://pubmed.ncbi.nlm.nih.gov/38498445/
Lu Y, Zhou Y, Nakai S, Hosomi M, Zhang H, Kronzucker HJ, Shi W. Stimulation of nitrogen removal in the rhizosphere of aquatic duckweed by root exudate components. Planta. 2014; 239(3):591-603. Available from: https://pubmed.ncbi.nlm.nih.gov/24271005/
Lu Y, Yuan T, Yun SH, Wang W, Wu Q, Kannan K. Occurrence of cyclic and linear siloxanes in indoor dust from China, and implications for human exposures. Environ Sci Technol. 2010;44(16):6081-7. Available from: https://pubmed.ncbi.nlm.nih.gov/20704203/
Petkowski JJ, Bains W, Seager S. On the Potential of Silicon as a Building Block for Life. Life (Basel). 2020;10(6):84. Available from: https://pubmed.ncbi.nlm.nih.gov/32532048/
Meeks RG, Stump DG, Siddiqui WH, Holson JF, Plotzke KP, Reynolds VL. An inhalation reproductive toxicity study of octamethylcyclotetrasiloxane (D4) in female rats using multiple and single day exposure regimens. Reprod Toxicol. 2007;23(2):192-201. Available from: https://pubmed.ncbi.nlm.nih.gov/17254748/
Burns-Naas LA, Meeks RG, Kolesar GB, Mast RW, Elwell MR, Hardisty JF, et al. Inhalation toxicology of octamethylcyclotetrasiloxane (D4) following a 3-month nose-only exposure in Fischer 344 rats. Int J Toxicol. 2002;21(1):39-53. Available from: https://pubmed.ncbi.nlm.nih.gov/11936898/
Burns-Naas LA, Mast RW, Klykken PC, McCay JA, White KL, et al. Toxicology and humoral immunity assessment of decamethylcyclopentasiloxane (D5) following a 1-month whole body inhalation exposure in Fischer 344 rats. Toxicol Sci. 1998;43(1):28-38. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1096608098924428
Zhang Y, Wang Z. Comparative analysis of essential oil components of two Pinus species from Taibai Mountain in China. Nat Prod Commun. 2010;5(8):1295-8. Available from: https://pubmed.ncbi.nlm.nih.gov/20839639/
Ankney E, Swor K, Satyal P, Setzer WN. Essential Oil Compositions of Pinus Species (P. contorta Subsp. contorta, P. ponderosa var. ponderosa, and P. flexilis); Enantiomeric Distribution of Terpenoids in Pinus Species. Molecules. 2022;27(17):5658. Available from: https://pubmed.ncbi.nlm.nih.gov/36080426/
Prashar P, Kapoor N, Sachdeva S. Rhizosphere: its structure, bacterial diversity and significance. Rev Environ Sci Biotechnol. 2014;13(1):63-77. Available from: https://link.springer.com/article/10.1007/s11157-013-9317-z
Pii Y, Mimmo T, Tomasi N, Terzano R, Cesco S, Crecchio C. Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review. Biol Fertil Soils. 2015;51(4):403-415. Available from: https://link.springer.com/article/10.1007/s00374-015-0996-1